EndoTrap® affinity chromatography is one principal method for cleaning biological solutions from endotoxin
contaminations. The EndoTrap® ligand is a protein by nature, which is bound to the polymeric bead-matrix by stable
covalent bonds. However, leakage of minute amounts of ligand is a matter of fact for all affinity materials and testing on
these contaminants is often required for regulatory purposes. Depending on the intended use of the preparation and the
step in the purification process (early or late), where EndoTrap® is used, a quantitative analysis of residual EndoTrap®
ligand might be required. The EndoTrap® HD Leakage ELISA was developed to allow an accurate and reproducible
determination of small amounts of EndoTrap® ligand in biological samples. This ELISA is suitable for detection of leached
ligand from EndoTrap® HD resin and EndoTrap® blue res