100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation. The ladder is generated from PCR and restriction enzyme digestion of double stranded DNA. Ready-to-Use Ladders are provided pre-diluted in 1X loading buffer at a concentration of 20 ng/uL. A separate vial of 6X loading buffer is provided to add to your DNA samples. The loading buffer provided contains density agents and two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.
产品描述
100 bp DNA Ladder适用于测定100 bp至1500 bp的线性双链DNA片段的大小。500 bp和1，500 bp条带含有更多的DNA以提供内部取向。梯状条带由PCR和双链DNA的限制性酶消化产生。即用型阶梯在1X上样缓冲液中以20 ng/uL的浓度预稀释。提供一个单独的6X上样缓冲液小瓶以添加到您的DNA样本中。提供的上样缓冲液含有密度剂和两种蓝色电泳跟踪染料，其在1%琼脂糖凝胶中以约1.5kb和200 bp运行。
