The oligomeric form of Amyloid Beta peptide (AB, 1-42) has been closely linked to Alzheimers Disease. Several ELISAs targeting AB have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al., 2012), has been shown to specifically detect AB, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of AB peptide (o-AB) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody.
The Biosensis oligomeric AB ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric AB peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-AB capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-AB present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric AB peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-AB standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
产品特点
该ELIAS试剂盒包含:1 x 96孔预包被条孔板(可拆卸), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.